Kluyveromyces lactis HIS4transcriptional regulation: similarities and differences toSaccharomyces cerevisiae HIS4gene
نویسندگان
چکیده
منابع مشابه
Regulation of lactose transport in Kluyveromyces lactis
Kluyveromyces lactis is able to ferment lactose and it is considered as an alternative model for biochemical, physiological and genetic studies in nonSaccharomyces yeasts. Understanding the mechanisms of lactose uptake and the regulation involved in the transport is an important step towards better biotechnological applications. The transport of lactose in K. lactis is mediated by a membrane pe...
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Utilization of nonfermentable carbon sources by Kluyveromyces lactis and Saccharomyces cerevisiae requires the Snf1p kinase and the Cat8p transcriptional activator, which binds to carbon source-responsive elements of target genes. We demonstrate that KlSnf1p and KlCat8p from K. lactis interact in a two-hybrid system and that the interaction is stronger with a kinase-dead mutant form of KlSnf1p....
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High expression and secretion of recombinant ovine beta-lactoglobulin has been achieved in the yeast Kluyveromyces lactis. The yield of beta-lactoglobulin is 40-50 mg per litre of culture supernatant and accounts for approx. 72% of the total secreted protein. Constitutive expression is under the control of the Saccharomyces cerevisiae phosphoglycerate kinase promoter from an intronless version ...
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The cryptic mating type loci in Saccharomyces cerevisiae act as reservoirs of mating type information used in mating type switching in homothallic yeast strains. The transcriptional silencing of these loci depends on the formation of a repressive chromatin structure that is reminiscent of heterochromatin. Silent information regulator (Sir) proteins 2-4 are absolutely required for silencing. To ...
متن کاملRandom exploration of the Kluyveromyces lactis genome and comparison with that of Saccharomyces cerevisiae.
The genome of the yeast Kluyveromyces lactis was explored by sequencing 588 short tags from two random genomic libraries (random sequenced tags, or RSTs), representing altogether 1.3% of the K. lactis genome. After systematic translation of the RSTs in all six possible frames and comparison with the complete set of proteins predicted from the Saccharomyces cerevisiae genomic sequence using an i...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1999
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(99)01105-9